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Once the script has been configured click **OK** to start the analysis. The script will display two tables (one for each channel with the given channel name) and the labeling
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output for the puncta within nuclear regions only.
Results of 3D nuclear puncta segmentation on the sample data.
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@@ -60,6 +61,29 @@ and a pixel width and height of 0.0650 μm with a step size of 0.1 μm (see the
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| Voxel depth (μm) | 0.1000 |
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+--------------------+---------+
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Labeling output and result tables
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---------------------------------
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After the script completes two results tables will be displayed, one for each channel respectively. Each table contains measurements for the **size** of the label, the **volume** and the **sphericity**. Both the volume and sphericity Ops
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work on mesh objects, while the size Op works on the sample itself.
The output result tables for the "Vif" and "Nuclei" channels.
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In addition to the result tables, the label imdage (also known as an *index image*) of the channel "A" data extracted from channel "B" regions is shown. Note that these labels are 3D (XYZ) and are used to create meshes for the geometry measurements.
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